intercellular cell adhesion molecule 1 icam 1 antibody  (Proteintech)

Journal: Frontiers in Physiology

Article Title: PRDM16 Upregulation Induced by MicroRNA-448 Inhibition Alleviates Atherosclerosis via the TGF-β Signaling Pathway Inactivation

doi: 10.3389/fphys.2020.00846

Figure Lengend Snippet: PRDM16 inhibited AS through inactivation of the TGF-β signaling pathway. (A) The content of TC, TG, and HDL-C in peripheral blood in mice in each group as detected by biochemical indicators. (B) The changes of aortic plaques in AS mice as detected by HE staining (×200, scale bar: 50 μm). (C) The number of aortic thrombosis in AS mice as detected by ORO staining (×400, scale bar: 25 μm). (D) Positive rates of AS markers (ICAM-1 and VCAM-1) in the aorta of AS mice as detected by IHC (×400). * p < 0.05 compared with mice treated with DMSO, # p < 0.05 compared with mice treated with oe-PRDM16 NC. The measurement data were expressed as mean ± standard deviation, and analyzed by independent-sample t -test, n = 15.

Article Snippet: The tissue sections were probed with primary antibodies to intercellular cell adhesion molecule-1 (ICAM-1) (#4915, 1:50, Cell Signaling Technology, Danvers, MA, United States) and vascular cell adhesion molecule-1 (VCAM-1) (ab134047, 1:250, Abcam, Cambridge, United Kingdom) at 4°C overnight.

Techniques: Staining, Standard Deviation